Highly Efficient and Uniform Single Stranded DNA Immobilization Using a Phase-Transfer Catalyst

Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase

DNA library preparation for high-throughput sequencing of genomic DNA usually involves ligation of adapters to double-stranded DNA fragments. However, for highly degraded DNA, especially ancient DNA, library preparation has been found to be more efficient if each of the two DNA strands are converted into library molecules separately. We present a new method for single-stranded library preparati...

Building a Modular Single-Stranded DNA Production Device Using Multi-Copy Single-Stranded DNA

A Novel Stand-Alone Single-Phase Induction Generator Using a Three-Phase Machine and a Single-Phase PWM Inverter

A new type of single-phase stand-alone induction generator using a three-phase induction machine and a single-phase voltage-source PWM inverter is introduced. The generator scheme is capable of producing constant load frequency with a very well regulated output voltage. A small lead acid battery and a single-phase full diode-bridge rectifier are used to feed the inverter. The inverter feeds one...

Nonenzymatic sequence-specific methyl transfer to single-stranded DNA.

2'-Deoxyuridine 5'-triphosphate with a methylthioether moiety at the 5 position can be incorporated into a primer-template DNA complex with Klenow enzyme. Activation with CNBr at 25 degrees C, pH 5.5, followed by treatment with piperidine produces sequence-specific cleavage on the template DNA predominantly at a single guanine position. The mechanism involves methyl-group transfer from sulfur o...

One-pot Synthesis of Pyrano[2,3-d]Pyrimidines using Nanocellulose-SbCl5 as a Highly Efficient and Bio-based Catalyst

The reaction of nano-cellulose and antimony pentachloridein dichloromethane gave nano-cellulose-SbCl5. Also nano-cellulose-SbCl5 has beencharacterized by energy dispersive X-ray spectroscopy(EDX), scanning electron microscopy (SEM)and Fourier transform infrared spectroscopy(FTIR).Nano-cellulose-SbCl5has been applied as a nano-catalyst for synthesis of pyrano[2,3-d]pyrimidines from the simple on...